Review



chang conjunctival cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC chang conjunctival cells
    Chang Conjunctival Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chang+conjunctival+cells/L+Cells/10__3390_slash_v16121827-99-0-39
    Average 99 stars, based on 4442 article reviews
    chang conjunctival cells - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Short Tandem Repeat (STR) Profiles of Commonly Used Human Ocular Surface Cell Lines
    Article Snippet: Notably the profile for the HCjE cells ( ) was distinct from that of Chang conjunctival cells (available at ATCC, https://www.atcc.org/STR%20Database.aspx?geo_country=usa ).

    Cell Culture:

    Article Title: In Vitro Corneal and Conjunctival Wound-Healing Assays as a Tool for Antiglaucoma Prostaglandin Formulation Characterization.
    Article Snippet: The cells were seeded in six-well culture dishes or on chamber slides (Lab-TekTM; Nalge Nunc International, Rochester, NY, USA) for the immunological analysis at a density of 100,000 cells per well and kept at 37 ◦C for 24 h. Cells were grown to reach a 90%–95% confluence in the center area of the well for the scraping assays before all the experiments [19]. .. The Wong-Kilbourne derivative of Chang conjunctival cells (clone 1 to 5c-4l American Type Culture Collection [ATCC, Manassas, VA]-certified cell line [CCL], 20.2) were cultured under standard conditions (humidified atmosphere of 5% CO2 at 37 ◦C) in a mixture of Dulbecco’s modified Eagle medium (Gibco, DMEM (1X), liquid, with 1000 mg/L D-glucose, sodium pyruvate with Lglutamine, 1000 mg/L D-glucose, sodium pyruvate) supplemented with 10% FBS, 50 IU/mL penicillin, and 50 IU/mL streptomycin. ..

    Article Title: Phosphosulindac is efficacious in an improved concanavalin A-based rabbit model of chronic dry eye disease.
    Article Snippet: Dry eye disease (DED), an inflammatory disease of the ocular surface, affects 15% of humans worldwide.. No satisfactory treatment exists for DED partly due to the lack of informative animal models of this disease.. We evaluated the anti-inflammatory phosphosulindac (PS) for the treatment of DED, using a new rabbit model of chronic DED.

    Modification:

    Article Title: In Vitro Corneal and Conjunctival Wound-Healing Assays as a Tool for Antiglaucoma Prostaglandin Formulation Characterization.
    Article Snippet: The cells were seeded in six-well culture dishes or on chamber slides (Lab-TekTM; Nalge Nunc International, Rochester, NY, USA) for the immunological analysis at a density of 100,000 cells per well and kept at 37 ◦C for 24 h. Cells were grown to reach a 90%–95% confluence in the center area of the well for the scraping assays before all the experiments [19]. .. The Wong-Kilbourne derivative of Chang conjunctival cells (clone 1 to 5c-4l American Type Culture Collection [ATCC, Manassas, VA]-certified cell line [CCL], 20.2) were cultured under standard conditions (humidified atmosphere of 5% CO2 at 37 ◦C) in a mixture of Dulbecco’s modified Eagle medium (Gibco, DMEM (1X), liquid, with 1000 mg/L D-glucose, sodium pyruvate with Lglutamine, 1000 mg/L D-glucose, sodium pyruvate) supplemented with 10% FBS, 50 IU/mL penicillin, and 50 IU/mL streptomycin. ..

    Eye Drops:

    Article Title: Ophthalmological formulations for the prevention of a coronavirus infection
    Article Snippet: .. Experimental series 1: A human corneal epithelial cell line (ATCC-CRL-11515 cells) and The Wong-Kilbourne derivative of Chang conjunctival cells (ATCC CCL-20.2) will be utilized to test the eye drops. ..

    Article Title: Ophthalmological formulations for the prevention of a coronavirus infection
    Article Snippet: .. Experimental series 2: The cytotoxicity of remdesivir eye drops will be assessed in a human corneal epithelial cell line (ATCC-CRL-11515 cells) and The Wong-Kilbourne derivative of Chang conjunctival cells (ATCC CCL-20.2) (see Ayaki M, et al., Clin Ophthalmol. ..



    Similar Products

    chang conjunctival cells  (ATCC)
    99
    ATCC chang conjunctival cells
    Chang Conjunctival Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chang+conjunctival+cells/L+Cells/10__3390_slash_v16121827-99-0-39
    Average 99 stars, based on 1 article reviews
    chang conjunctival cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    hadv d37 employed chang conjunctival cells  (ATCC)
    99
    ATCC hadv d37 employed chang conjunctival cells
    Ad64.eGFP delivers GFP gene via CD46. ( A ) Pre-treatment of HeLa cells with 10 mU neuraminidase from Vibrio cholerae to remove cell surface sialic acid or 10 mM EGTA to chelate calcium ions prior to incubation with Ad64.eGFP significantly decreased susceptibility to viral entry as determined by cell fluorescence compared to uninfected cells (Analysis of variance followed by Tukey’s test (ANOVA-T), n = 3 for each treatment). Error bars represent standard deviations. ( B ) <t>HAdV-B16</t> fiber knob (16 FK) blocked Ad5 pseudotyped with Ad16 fiber (Ad5.F16) and HAdV-D64 (Ad64), but not HAdV-C5 (Ad5) gene delivery into HeLa cells in a concentration-dependent manner. Experiments were performed in duplicate. ( C ) Anti-CD46 antibody blocked calcium-dependent entry into HeLa cells (ANOVA-T, n = 3 for each treatment). ( D ) Expression of CD46 BC1 or C1 isoform on CHO cells conferred susceptibility to Ad64.eGFP entry (ANOVA-T, n = 3 for each treatment). Single asterisk, p < 0.05; double asterisk, p < 0.0001.
    Hadv D37 Employed Chang Conjunctival Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chang+conjunctival+cells/L+Cells/pmc11680334-140-4-26
    Average 99 stars, based on 1 article reviews
    hadv d37 employed chang conjunctival cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    chang human conjunctival epithelial (hce) cell line  (European Collection of Authenticated Cell Cultures)
    90
    European Collection of Authenticated Cell Cultures chang human conjunctival epithelial (hce) cell line
    Ad64.eGFP delivers GFP gene via CD46. ( A ) Pre-treatment of HeLa cells with 10 mU neuraminidase from Vibrio cholerae to remove cell surface sialic acid or 10 mM EGTA to chelate calcium ions prior to incubation with Ad64.eGFP significantly decreased susceptibility to viral entry as determined by cell fluorescence compared to uninfected cells (Analysis of variance followed by Tukey’s test (ANOVA-T), n = 3 for each treatment). Error bars represent standard deviations. ( B ) <t>HAdV-B16</t> fiber knob (16 FK) blocked Ad5 pseudotyped with Ad16 fiber (Ad5.F16) and HAdV-D64 (Ad64), but not HAdV-C5 (Ad5) gene delivery into HeLa cells in a concentration-dependent manner. Experiments were performed in duplicate. ( C ) Anti-CD46 antibody blocked calcium-dependent entry into HeLa cells (ANOVA-T, n = 3 for each treatment). ( D ) Expression of CD46 BC1 or C1 isoform on CHO cells conferred susceptibility to Ad64.eGFP entry (ANOVA-T, n = 3 for each treatment). Single asterisk, p < 0.05; double asterisk, p < 0.0001.
    Chang Human Conjunctival Epithelial (Hce) Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chang+conjunctival+cells/chang+human+conjunctival+epithelial++hce++cell+line/pm36430590-280-1-25
    Average 90 stars, based on 1 article reviews
    chang human conjunctival epithelial (hce) cell line - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    chang human conjunctival epithelial hce cell line  (ATCC)
    94
    ATCC chang human conjunctival epithelial hce cell line
    Figure 1. Bimatoprost (BIM) 0.01% does not induce changes in human <t>conjunctival</t> <t>epithelial</t> <t>(HCE)</t> cells proliferation and viability. Cell growth (A) and viability (B) rate in control (vCTR) and treated HCE cells incubated for 24 and 48 h with BIM 0.01% and 0.03%. BIM 0.03% induces a significant decrease in cell growth and viability at 24 and 48 h. Results are the media of three independent experiments ± SE; one-way ANOVA (Dunnett’s method); * p < 0.05 compared with vCTR.
    Chang Human Conjunctival Epithelial Hce Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chang+conjunctival+cells/clone+1-5c-4%3B+HeLa+Contaminant%3B+Human/pm36430590-280-5-14
    Average 94 stars, based on 1 article reviews
    chang human conjunctival epithelial hce cell line - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Ad64.eGFP delivers GFP gene via CD46. ( A ) Pre-treatment of HeLa cells with 10 mU neuraminidase from Vibrio cholerae to remove cell surface sialic acid or 10 mM EGTA to chelate calcium ions prior to incubation with Ad64.eGFP significantly decreased susceptibility to viral entry as determined by cell fluorescence compared to uninfected cells (Analysis of variance followed by Tukey’s test (ANOVA-T), n = 3 for each treatment). Error bars represent standard deviations. ( B ) HAdV-B16 fiber knob (16 FK) blocked Ad5 pseudotyped with Ad16 fiber (Ad5.F16) and HAdV-D64 (Ad64), but not HAdV-C5 (Ad5) gene delivery into HeLa cells in a concentration-dependent manner. Experiments were performed in duplicate. ( C ) Anti-CD46 antibody blocked calcium-dependent entry into HeLa cells (ANOVA-T, n = 3 for each treatment). ( D ) Expression of CD46 BC1 or C1 isoform on CHO cells conferred susceptibility to Ad64.eGFP entry (ANOVA-T, n = 3 for each treatment). Single asterisk, p < 0.05; double asterisk, p < 0.0001.

    Journal: Viruses

    Article Title: CD46 Is a Protein Receptor for Human Adenovirus Type 64

    doi: 10.3390/v16121827

    Figure Lengend Snippet: Ad64.eGFP delivers GFP gene via CD46. ( A ) Pre-treatment of HeLa cells with 10 mU neuraminidase from Vibrio cholerae to remove cell surface sialic acid or 10 mM EGTA to chelate calcium ions prior to incubation with Ad64.eGFP significantly decreased susceptibility to viral entry as determined by cell fluorescence compared to uninfected cells (Analysis of variance followed by Tukey’s test (ANOVA-T), n = 3 for each treatment). Error bars represent standard deviations. ( B ) HAdV-B16 fiber knob (16 FK) blocked Ad5 pseudotyped with Ad16 fiber (Ad5.F16) and HAdV-D64 (Ad64), but not HAdV-C5 (Ad5) gene delivery into HeLa cells in a concentration-dependent manner. Experiments were performed in duplicate. ( C ) Anti-CD46 antibody blocked calcium-dependent entry into HeLa cells (ANOVA-T, n = 3 for each treatment). ( D ) Expression of CD46 BC1 or C1 isoform on CHO cells conferred susceptibility to Ad64.eGFP entry (ANOVA-T, n = 3 for each treatment). Single asterisk, p < 0.05; double asterisk, p < 0.0001.

    Article Snippet: Previous molecular studies on HAdV-D37 employed Chang conjunctival cells [ , , , ], which were later shown to be contaminated with HeLa cells during establishment (ATCC), or A549 lung epithelial cells [ , ].

    Techniques: Incubation, Fluorescence, Concentration Assay, Expressing

    Direct binding of HAdV-D64 to soluble CD46. ( A ) Ad64.eGFP particles were co-precipitated with His-tagged soluble CD46 (sC) using Ni-NTA Agarose then subject to SDS-PAGE. Main panel lanes: 1, CD46 (~45–50 kDa) mixed with Ad64.eGFP; 2, flowthrough from NiNTA Agarose, 3–5 wash fractions, 6–8 elution fractions. Hx, HAdV-D64 hexon. Gel images of ultracentrifugation-purified Ad64.eGFP (top left) and purified sCD46-C (bottom left) are shown to the left for comparison. Masses of molecular standards are shown in kDa. ( B ) 5 nm Ni-NTA-NanoGold beads (Au) were incubated with CD46, Ad64.eGFP particles, or both, filtered through a 0.1 µm filter, visualized using transmission electron microscopy at 60,000× magnification, and counted. Only the presence of the large adenovirus particle and CD46 led to substantial retention of NanoGold beads in the filter (ANOVA-T, n = 3 for each treatment). Double asterisks, p < 0.0001.

    Journal: Viruses

    Article Title: CD46 Is a Protein Receptor for Human Adenovirus Type 64

    doi: 10.3390/v16121827

    Figure Lengend Snippet: Direct binding of HAdV-D64 to soluble CD46. ( A ) Ad64.eGFP particles were co-precipitated with His-tagged soluble CD46 (sC) using Ni-NTA Agarose then subject to SDS-PAGE. Main panel lanes: 1, CD46 (~45–50 kDa) mixed with Ad64.eGFP; 2, flowthrough from NiNTA Agarose, 3–5 wash fractions, 6–8 elution fractions. Hx, HAdV-D64 hexon. Gel images of ultracentrifugation-purified Ad64.eGFP (top left) and purified sCD46-C (bottom left) are shown to the left for comparison. Masses of molecular standards are shown in kDa. ( B ) 5 nm Ni-NTA-NanoGold beads (Au) were incubated with CD46, Ad64.eGFP particles, or both, filtered through a 0.1 µm filter, visualized using transmission electron microscopy at 60,000× magnification, and counted. Only the presence of the large adenovirus particle and CD46 led to substantial retention of NanoGold beads in the filter (ANOVA-T, n = 3 for each treatment). Double asterisks, p < 0.0001.

    Article Snippet: Previous molecular studies on HAdV-D37 employed Chang conjunctival cells [ , , , ], which were later shown to be contaminated with HeLa cells during establishment (ATCC), or A549 lung epithelial cells [ , ].

    Techniques: Binding Assay, SDS Page, Purification, Comparison, Incubation, Transmission Assay, Electron Microscopy

    HAdV-D64 can enter human conjunctival epithelial cells. ( A ) Pre-treatment of HCjE cells with soluble CD46-C (sCD46) and/or neuraminidase inhibited Ad64.eGFP viral entry, as determined by expression of green fluorescence of eGFP transgene (ANOVA-T, n = 5). ( B ) Ad64.eGFP vector entry into HCjE cells in Keratinocyte-SFM in the presence or absence of EGTA, a calcium chelator (ANOVA-T, n = 3). ** denotes p < 0.001.

    Journal: Viruses

    Article Title: CD46 Is a Protein Receptor for Human Adenovirus Type 64

    doi: 10.3390/v16121827

    Figure Lengend Snippet: HAdV-D64 can enter human conjunctival epithelial cells. ( A ) Pre-treatment of HCjE cells with soluble CD46-C (sCD46) and/or neuraminidase inhibited Ad64.eGFP viral entry, as determined by expression of green fluorescence of eGFP transgene (ANOVA-T, n = 5). ( B ) Ad64.eGFP vector entry into HCjE cells in Keratinocyte-SFM in the presence or absence of EGTA, a calcium chelator (ANOVA-T, n = 3). ** denotes p < 0.001.

    Article Snippet: Previous molecular studies on HAdV-D37 employed Chang conjunctival cells [ , , , ], which were later shown to be contaminated with HeLa cells during establishment (ATCC), or A549 lung epithelial cells [ , ].

    Techniques: Expressing, Fluorescence, Plasmid Preparation

    Figure 1. Bimatoprost (BIM) 0.01% does not induce changes in human conjunctival epithelial (HCE) cells proliferation and viability. Cell growth (A) and viability (B) rate in control (vCTR) and treated HCE cells incubated for 24 and 48 h with BIM 0.01% and 0.03%. BIM 0.03% induces a significant decrease in cell growth and viability at 24 and 48 h. Results are the media of three independent experiments ± SE; one-way ANOVA (Dunnett’s method); * p < 0.05 compared with vCTR.

    Journal: International journal of molecular sciences

    Article Title: Cytotoxicity, Mitochondrial Functionality, and Redox Status of Human Conjunctival Cells after Short and Chronic Exposure to Preservative-Free Bimatoprost 0.03% and 0.01%: An In Vitro Comparative Study.

    doi: 10.3390/ijms232214113

    Figure Lengend Snippet: Figure 1. Bimatoprost (BIM) 0.01% does not induce changes in human conjunctival epithelial (HCE) cells proliferation and viability. Cell growth (A) and viability (B) rate in control (vCTR) and treated HCE cells incubated for 24 and 48 h with BIM 0.01% and 0.03%. BIM 0.03% induces a significant decrease in cell growth and viability at 24 and 48 h. Results are the media of three independent experiments ± SE; one-way ANOVA (Dunnett’s method); * p < 0.05 compared with vCTR.

    Article Snippet: The Wong–Kilbourne derivative of the Chang human conjunctival epithelial (HCE) cell line (obtained from ATCC®, CCL 20.2; clone 1-5c-4; WKD; ChWK) was purchased by the European Collection of Authenticated Cell Cultures (ECACC 88021103).

    Techniques: Control, Incubation